Synthesis of a photosystem I polypeptide of 15 kilodaltons in isolated etiochloroplasts of wheat.
نویسنده
چکیده
Etiochloroplasts isolated from greening wheat (Triticum aestivum L. cv Norin 61) seedlings synthesized a membrane polypeptide of 15 kilodaltons. One-dimensional peptide mapping with Staphylococcus aureus V8 protease revealed that the 15 kilodaltons polypeptide is the subunit 5 of photosystem I reaction center complex.
منابع مشابه
Synthesis and Assembly of the Polypeptides of Photosystem I and II in Isolated Etiochloroplasts of Wheat.
Synthesis and assembly of photosystems (PS) I and II polypeptides in etiochloroplasts isolated from greening wheat (Triticum aestivum L. cv Norin 61) seedlings were studied. The isolated etiochloroplasts synthesized PSI polypeptides of 66 and 15 kilodaltons, PSII polypeptides of 46 and 42 kilodaltons, and atrazine-binding 34 to 32 kilodalton polypeptide. Their assembly processes in the thylakoi...
متن کاملSynthesis and Uptake of Cytoplasmically Synthesized Pyruvate
Polyadenylated RNA was isolated from maize leaves and translated in vitro. In agreement with a previous report by others, we found among the translation products a I 10-kilodalton pyruvate orthophosphate dikinase (PPDK) precursor that is about 16 kilodaltons larger than the polypeptide isolated from cells. This maize PPDK precursor polypeptide was taken up from the translation product mixture b...
متن کاملTopography of the protein complexes of the chloroplast thylakoid membrane : studies of photosystem I using a chemical probe and proteolytic digestion.
The transverse heterogeneity of the polypeptides associated with the Photosystem I (PSI) complex in spinach thylakoid membranes and in a highly resolved PSI preparation has been studied using the impermeant chemical modifier, 2,4,6-trinitrobenzenesulfonate (TNBS) and the proteolytic enzyme, Pronase E. The present study has shown that the PSI reaction center polypeptide of approximately 62 kilod...
متن کاملDevelopment of Photosystem I and Photosystem II Activities in Leaves of Light-grown Maize (Zea mays).
To compare chloroplast development in a normally grown plant with etiochloroplast development, green maize plants (Zea mays), grown under a diurnal light regime (16-hour day) were harvested 7 days after sowing and chloroplast biogenesis within the leaf tissue was examined. Determination of total chlorophyll content, ratio of chlorophyll a to chlorophyll b, and O(2)-evolving capacity were made f...
متن کاملThylakoid polypeptides of light and dark aged chloroplasts.
Spinach (Spinacia oleracea) chloroplasts were aged at 4 degrees C under red light and in the dark. The electron transport activity was monitored together with the thylakoid polypeptide patterns in sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The light-induced decay of photosystem II (PSII) activity (half-life, about 4 hours) was correlated with a decrease in polypeptides with appa...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- Plant physiology
دوره 81 2 شماره
صفحات -
تاریخ انتشار 1986